Dr. Matthias Klein

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Dr. Matthias Klein

Assistant Professor

klein.663@osu.edu

http://www.kleinomicslab.com/

614-688-1851

Office:

313 Parker Food Science and Technology Building

2015 Fyffe Road

Professional Information

Biography:

Dr. Klein earned his Diplom in Physics (equivalent to an MSc) at the University of Regensburg, Germany, and pursued his PhD in the field of Metabolomics at the same institution. During his studies, he discovered small molecule biomarkers predicting the onset of human liver and kidney diseases as well as ketosis in dairy cows. Dr. Klein held Postdoctoral positions at the Ludwig-Maximilians-Universität Munich, Germany, and the University of Calgary, Canada, where his focus turned toward microbial metabolism and microbiome-host interactions. In September 2017, Dr. Klein joined The Ohio State University’s Department of Food Science and Technology as an Assistant Professor within the Foods for Health Discovery Theme.
Dr. Klein is employing state-of-the-art technologies such as Nuclear Magnetic Resonance (NMR) and Liquid Chromatography-Mass Spectrometry (LC-MS) for the analysis of small molecule metabolites. Data from these platforms is analyzed using high-dimensional computational and statistical approaches employing software such as R and Matlab. Goals of this research are furthering the understanding of disease development and progression as well as the discovery of predictive disease biomarkers. Dr. Klein’s current research deals with the detection and identification of pathogenic microbes as well as metabolomics analyses on maternal health.

Area of Expertise: Metabolomics research on human and animal diseases and detection / identification of microbial infection. Methods include Nuclear Magnetic Resonance Spectroscopy, Computational Data Analysis and Statistics.

Works

Journal Articles

Edwards, K.M.; Badiger, A.; Heldman, D.R.; Klein, M.S., "Metabolomic Markers of Storage Temperature and Time in Pasteurized Milk" Metabolites 11 7 (2021) 419-419-419-419

Shearer, J.; Klein, M.S.; Vogel, H.J.; Mohammad, S. et al., "Maternal and Cord Blood Metabolite Associations with Gestational Weight Gain and Pregnancy Health Outcomes" Journal of Proteome Research 20 3 (2021) 1630-1638-1630-1638

Klein, M.S., "Affine Transformation of Negative Values for NMR Metabolomics Using the mrbin R Package." Journal of Proteome Research 20 2 (2021) 1397-1404-1397-1404

Huck, D.T.; Klein, M.S.; Meuti, M.E., "Determining the Effects of Nutrition on the Reproductive Physiology of Male Mosquitoes" Journal of Insect Physiology 129 (2021) 104191-104191-104191-104191

Wang, D.; Greenwood, P.; Klein, M.S., "A Protein-free Chemically Defined Medium for the Cultivation of Various Microorganisms with Food Safety Significance" Journal of Applied Microbiology 131 2 (2021) 844-854-844-854

Shearer, J.; Reimer, R.A.; Hittel, D.S.; Gault, M.A. et al., "Caffeine-Containing Energy Shots Cause Acute Impaired Glucoregulation in Adolescents" Nutrients 12 12 (2020) 3850-3850-3850-3850

Lee, A.H.; Dhingra, S.K.; Lewis, I.A.; Singh, M.K.; Siriwardana, A.; Dalal, S.; Rubiano, K.; Klein, M.S.; Baska, K.S.; Krishna, S.; Klemba, M. et al., "Evidence for Regulation of Hemoglobin Metabolism and Intracellular Ionic Flux by the Plasmodium falciparum Chloroquine Resistance Transporter" Scientific Reports 8 1 (2018) 13578-13578-13578-13578

Newell, C.; Sabouny, R.; Hittel, D.S.; Shutt, T.E.; Khan, A.; Klein, M.S.; Shearer, J., "Mesenchymal Stem Cells Shift Mitochondrial Dynamics and Enhance Oxidative Phosphorylation in Recipient Cells" Frontiers in Physiology 9 (2018) 1572-1572-1572-1572

Schober, D.; Jacob, D.; Wilson, M.; Cruz, J.A.; Marcu, A.; Grant, J.R.; Moing, A.; Deborde, C.; de Figueiredo, L.F.; Haug K.; Rocca-Serra, P.; Easton, J.; Ebbels, T.M.D.; Hao, J.; Ludwig, C. et al., "nmrML: A Community Supported Open Data Standard for the Description, Storage, and Exchange of NMR Data." Analytical Chemistry 90 1 (2018) 649-656-649-656

Newell, C.; Johnsen, V.L.; Yee, N.C.; Xu, W.J.; Klein, M.S.; Khan, A.; Rho, J.M.; Shearer, J., "Ketogenic Diet Leads to O-GlcNAc Modification in the BTBRT+tf/j Mouse Model of Autism." Biochimica et Biophysica Acta - Molecular Basis of Disease 1863 9 (2017) 2274-2281-2274-2281

Lu, W.; Su, X.; Klein, M.S.; Lewis, I.A. et al., "Metabolite Measurement: Pitfalls to Avoid and Practices to Follow" Annual Review of Biochemistry 86 1 (2017) 277-304-277-304

Beatty, P.; Klein, M.; Fischer, J.; Lewis, I. et al., "Understanding Plant Nitrogen Metabolism through Metabolomics and Computational Approaches" Plants 5 4 (2016) 39-39-39-39

Klein, M.S.; Shearer, J., "Metabolomics and Type 2 Diabetes: Translating Basic Research into Clinical Application" Journal of Diabetes Research 2016 (2016) 3898502-3898502-3898502-3898502

Klein, M.S.; Newell, C.; Bomhof, M.R.; Reimer, R.A.; Hittel, D.S.; Rho, J.M.; Vogel, H.J.; Shearer, J., "Metabolomic Modelling to Monitor Host Responsiveness to Gut Microbiota Manipulation in the BTBR T+tf /j Mouse" Journal of Proteome Research 15 (2016) 1143-1150-1143-1150

Tetens, J.; Heuer, C.; Heyer, I.; Klein, M.S.; Gronwald, W.; Junge, W.; Oefner, P.J.; Thaller, G.; Krattenmacher, N., "Polymorphisms Within the APOBR Gene are Highly Associated With Milk Levels of Prognostic Ketosis Biomarkers in Dairy Cows" Physiological Genomics 47 4 (2015) 129-137-129-137

Deschamps, C.L.; Connors, K.E.; Klein, M.S.; Johnsen, V.L.; Shearer, J.; Vogel, H.J.; Devaney, J.M.; Gordish-Dressman, H.; Many, G.M.; Barfield, W. et al., "The ACTN3 R577X Polymorphism Is Associated with Cardiometabolic Fitness in Healthy Young Adults" PLoS One 10 6 (2015) e0130644-e0130644-e0130644-e0130644

Ehret, A.; Hochstuhl, D.; Krattenmacher, N.; Tetens, J. et al., "Short Communication: Use of Genomic and Metabolic Information As Well as Milk Performance Records for Prediction of Subclinical Ketosis Risk via Artificial Neural Networks" Journal of Dairy Science 98 1 (2015) 322-329-322-329

Klein, M.S.; Connors, K.E.; Shearer, J.; Vogel, H.J. et al., "Metabolomics Reveals the Sex-Specific Effects of the SORT1 Low-Density Lipoprotein Cholesterol Locus in Healthy Young Adults" Journal of Proteome Research 13 11 (2014) 5063-5070-5063-5070

Klein, M.S.; Almstetter, M.F.; Nuernberger, N.; Sigl, G.; Gronwald, W.; Wiedemann, S.; Dettmer, K.; Oefner, P.J., "Correlations between Milk and Plasma Levels of Amino and Carboxylic Acids in Dairy Cows" Journal of Proteome Research 12 11 (2013) 5223-5232-5223-5232

Zacharias, H.; Hochrein, J.; Klein, M.; Samol, C. et al., "Current Experimental, Bioinformatic and Statistical Methods used in NMR Based Metabolomics" Current Metabolomics 1 3 (2013) 253-268-253-268

Zacharias, H.U.; Schley, G.; Hochrein, J.; Klein, M.S.; Koeberle, C.; Eckardt, K.U.; Willam, C.; Oefner, P.J.; Gronwald, W., "Analysis of Human Urine Reveals Metabolic Changes Related to the Development of Acute Kidney Injury Following Cardiac Surgery" Metabolomics 9 3 (2013) 697-707-697-707

Klein, M.S.; Oefner, P.J.; Gronwald, W., "MetaboQuant: A Tool Combining Individual Peak Calibration and Outlier Detection for Accurate Metabolite Quantification in 1D (1)H and (1)H-(13)C HSQC NMR Spectra" BioTechniques 54 5 (2013) 251-256-251-256

Thomas, A.; Klein, M.S.; Stevens, A.P.; Reinders, Y.; Hellerbrand, C.; Dett, K.; Gronwald, W.; Oefner, P.J.; Reinders, J., "Changes in the Hepatic Mitochondrial and Membrane Proteome in Mice Fed a Non-alcoholic Steatohepatitis Inducing Diet" Journal of Proteomics 80 (2013) 107-122-107-122

Hochrein, J.; Klein, M.S.; Zacharias, H.U.; Li, J.; Wijffels, G.; Schirra, H. R.; Oefner, P.J.; Gronwald, W., "Performance Evaluation of Algorithms for the Classification of Metabolic 1H NMR Fingerprints" Journal of Proteome Research 11 12 (2012) 6242-6251-6242-6251

Kohl, S.M.; Klein, M.S.; Hochrein, J.; Oefner, P.J. et al., "State-of-The Art Data Normalization Methods Improve NMR-Based Metabolomic Analysis" Metabolomics 8 (2012) 146-160-146-160

Thomas, A.; Stevens, A.P.; Klein, M.S.; Hellerbrand, C.; Dettmer, K.; Gronwald, W.; Oefner, P.J.; Reinders, J., "Early Changes in the Liver-Soluble Proteome From Mice Fed a Nonalcoholic Steatohepatitis Inducing Diet" Proteomics 12 9 (2012) 1437-1451-1437-1451

Klein, M.S.; Buttchereit, N.; Miemczyk, S.P.; Immervoll, A-K.; Louis, C.; Wiedemann, S.; Junge, W.; Thaller, G.; Oefner, P.J.; Gronwald, W., "NMR Metabolomic Analysis of Dairy Cows Reveals Milk Glycerophosphocholine to Phosphocholine Ratio As Prognostic Biomarker for Risk of Ketosis" Journal of Proteome Research 11 2 (2012) 1373-1381-1373-1381

Klein, M.S.; Dorn, C.; Saugspier, M.; Hellerbrand, C. et al., "Discrimination of Steatosis and NASH in Mice Using Nuclear Magnetic Resonance Spectroscopy" Metabolomics 7 2 (2011) 237-246-237-246

Gronwald, W.; Klein, M.S.; Zeltner, R.; Schulze, B-D.; Reinhold, S.W.; Deutschmann, M.; Immervoll, A.-K.; Boeger, C.A. et al., "Detection of Autosomal Dominant Polycystic Kidney Disease by NMR Spectroscopic Fingerprinting of Urine" Kidney International 79 11 (2011) 1244-1253-1244-1253

Klein, M.S.; Almstetter, M.F.; Schlamberger, G.; Nuernberger, N.; Dettmer, K.; Oefner, P.J.; Meyer, H.H.D.; Wiedeman, S.; Gronwald, W., "Nuclear Magnetic Resonance and Mass Spectrometry-Based Milk Metabolomics in Dairy Cows During Early and Late Lactation" Journal of Dairy Science 93 4 (2010) 1539-1550-1539-1550

Gronwald, W.; Klein, M.S.; Kaspar, H.; Fagerer, S.R.; Nuernberger, N.; Dettmer, K.; Bertsch, T.; Oefner, P.J., "Urinary Metabolite Quantification Employing 2D NMR Spectroscopy" Analytical Chemistry 80 23 (2008) 9288-9297-9288-9297

Patents

Invention Disclosure: Method for Removing Sodium from Food Products. Filed Jul 2019

Invention Disclosure: Method for Removing Inorganic Salt from Biological Metabolite Mixtures. Filed May 2019

Presentations

"Current Research from the Klein Lab." In FST seminar. 2020.

"Metabolomics: A Powerful Approach for Biological Research." In Department of Entomology seminar. 2020.

"Metabolomics for Human Health Research." In Center for Advanced Functional Food Research & Entrepreneurship (CAFFRE) seminar. 2018.

"Application of Metabolomics for Nutrition and Health Research." In Human Nutrition seminar. 2018.

"Data Analysis Approaches in Metabolomics of Human Disease and Microbial Metabolism." In Biomedical Informatics (BMI) seminar. 2018.

"Metabolomics Research on Human Chronic Disease and Microbial Contributors." In Diabetes & Metabolism Research Center (DMRC) seminar. 2018.

"Applications of Metabolomics in Human, Animal and Plant Research." In Center for Applied Plant Sciences (CAPS) seminar. 2018.

"Metabolomics for Rapid and Accurate Pathogen Identification." In FST seminar. 2017.

"Multidimensional Approaches in NMR Metabolomics." In NMR@OSU seminar. 2017.

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Dr. Matthias Klein

Profile Tabs

Professional Information

Works

Journal Articles

Patents

Presentations